In bioprocess operations, the UV absorbance of a liquid solution can identify the absence or presence of the molecule of interest. The measurement, typically at 280nm, is made by a spectrophotometer or photometer either in-line or off-line in a cuvette. A collimated beam of light passes through a sample with a defined path length and the absorbance is determined as the ratio of the light applied from the source to what passed through the sample.

The Single Use UV Flow Cell enables the measurement to be made non-invasively. The flow cell is connected to tubing, and the measurement is made by use of a compact photometer with fiber optic cables. This flow cell contains a special silica glass lens on the wall and compartments to attach the light source and detector. The stream to be measured flows between the lenses by way of tubing attached to the hose barb ends of the flow cell.

Technical sheet UV Absorbance Sensors